Anti-Acanthamoeba efficacy of the multipurpose contact lens disinfecting solutions

摘要

? 2016 Japan Health Sciences University & Japan International Cultural Exchange Foundation. ? 2016 Japan Health Sciences University & Japan International Cultural Exchange Foundation. Introduction: Disinfections of contact lens play a crucial part in eliminating Acanthamoeba keratitis. This study was therefore performed to evaluate the effectiveness of contact lens disinfecting solutions on cysts of Acanthamoeba castellani derived clinically from keratitis case based on the exposure times suggested by manufacturers, 4 hours and 8 hours. Materials and Methods: Three brands of multipurpose contact lens disinfecting solutions currently available in the market consisting of Freshlook All in One TM , Solo Care? Plusc and Renu Multi-Plus? were tested. Suspension of cysts was exposed to the disinfecting solutions at intervals varying from 5 minutes to 8 hours and later cultured onto non-nutrient agar lawned with Escherichia coli for the growth of Acanthamoeba trophozoites. Results: All three multipurpose contact lens disinfecting solutions (Freshlook All in One TM , Solo Care? Plusc and Renu Multi-Plus?) showed no effect on Acanthamoeba castellani cysts in time suggested by the manufacturers, 4 hours, and 8 hours. Conclusion: This study has proven that though contact lens disinfecting solutions have antimicrobial properties, not all are effective against Acanthamoeba cysts. The search for a solution which is effective against Acanthamoeba cysts would be desirable for contact lens wearers. Introduction: Disinfections of contact lens play a crucial part in eliminating Acanthamoeba keratitis. This study was therefore performed to evaluate the effectiveness of contact lens disinfecting solutions on cysts of Acanthamoeba castellani derived clinically from keratitis case based on the exposure times suggested by manufacturers, 4 hours and 8 hours. Materials and Methods: Three brands of multipurpose contact lens disinfecting solutions currently available in the market consisting of Freshlook All in One TM TM , Solo Care? Plusc and Renu Multi-Plus? were tested. Suspension of cysts was exposed to the disinfecting solutions at intervals varying from 5 minutes to 8 hours and later cultured onto non-nutrient agar lawned with Escherichia coli for the growth of Acanthamoeba trophozoites. Results: All three multipurpose contact lens disinfecting solutions (Freshlook All in One TM TM , Solo Care? Plusc and Renu Multi-Plus?) showed no effect on Acanthamoeba castellani cysts in time suggested by the manufacturers, 4 hours, and 8 hours. Conclusion: This study has proven that though contact lens disinfecting solutions have antimicrobial properties, not all are effective against Acanthamoeba cysts. The search for a solution which is effective against Acanthamoeba cysts would be desirable for contact lens wearers.