Characterization of Chinese Grape Seed Oil by Physicochemical Properties, Fatty Acid Composition, Triacylglycrol Profiles, and Sterols and Squalene Composition

摘要

This work focused on physicochemical property assaying, fatty acid composition, triacylglycerol profiles, and sterols and squalene composition of the Chinese grape seed oil. Three different methods were used to extract oil from Chinese grape seeds, solvent extraction, cold press and hot press methods. Three oils all possess very low acid value and peroxide value, suggesting their good quality as edible oil. Fatty acid composition results indicated that the total content of unsaturated fatty acids was 89.14 %, within which the majority fatty acid was linoleic acid with content of 64.11 %. Characterization of triacylglycerol profiles was achieved by a two-dimensional HPLC coupling of nonaqueous reverse-phase and silver ion HPLC with atmospheric pressure chemical ionization mass spectrometry method. There was a total of 30 TAGs including 23 regioisomers that have been determined. Unsaponifiable matters composition results revealed that the total sterols content and the squalene content were 278.49 mg/100 g and 17.81 mg/100 g, respectively.