Application of Genomic In Situ Hybridization in Horticultural Science

摘要

Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure andfunction, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, andpolyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploidsof various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the smalland numerous chromosomes in fruit species, for example, Diospyros hybrids. This analytical technique has proved to be the mostexact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybridssuch as Clivia, Rhododendron, and Lycoris ornamental hybrids. The genome characterization facilitates in hybrid selection havingpotential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressedsequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH)techniques in horticultural plants.