Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography

摘要

A reliable and rapidmethod has been developed for the determination of aflatoxin B1 (AFB1) in four kinds of feedstuffs comprising broken rice, peanuts, corn, and fishmeal.A sample preparation was carried out based on the QuEChERS method with the exclusion of the clean-up step.In this study, AFB1 was extracted using acetonitrile/methanol (40/60 v/v), followed by partitioning with sodium chloride and magnesium sulfate.High-performance liquid chromatography with precolumn derivatization and fluorescence detection was performed.The coefficients of determination were greater than 0.9800.Throughout the developed method, the recovery of all feedstuffs achieved a range of 82.50-109.85% with relative standard deviation lower than 11% for all analytes at a concentration of 20-100 ng/g.The limit of detection (LOD) ranged from 0.2 to 1.2 ng/g and limit of quantitation (LOQ) ranged from0.3 to 1.5 ng/g.The validatedmethod was successfully applied to a total of 120 samples.The occurrence of AFB1 contamination was found at the following concentrations: in broken rice (0.44-2.33ng/g), peanut (3.97-106.26ng/g), corn (0.88- 50.29 ng/g), and fishmeal (1.06-10.35ng/g).These results indicate that the proposed methodmay be useful for regularlymonitoring AFB1 contamination in feedstuffs.