固相萃取-高效液相色谱法测定啤酒原辅料中黄曲霉毒素B1

摘要

By comparing the recoveries of different solid phase extraction columns for aflatoxin Bl （ AFB1 ） clean- up, the silicone-diatomite-neutral alumina mixed column was chosen, and the analytical method of Reversed Phase High Performance Liquid Chromatography （RP-HPLC） was developed for determination of aflatoxin B1 in raw materi- als of beer （barley malt, barley, rice and wheat malt）. The results showed that the linear range of detection of AFBt ranged from 0.5μg/kg to 50 μg/kg. Standard curve correlation coefficient（r） was 0. 9997. The detection limit was 0. 15μg/kg. The average recovery ranged from 80. 1% to 109.2%, and the relative standard deviation（RSD） ranged from 0.23% to 4.29%. The method was simple, accurate and cost saving. It was also proposed to be used for the quality control of AFB1 in raw materials of beer.%以啤酒酿造中主要原辅料（大麦麦芽、大麦、大米、小麦麦芽）为样品，比较了几种不同净化柱净化黄曲霉毒素B1（AFB1）的回收率，最终选择了硅胶-硅藻土-中性氧化铝混合柱作为净化柱，建立了一种检测啤酒原辅料中AFB。的反相高效液相色谱（RP—HPLC）方法。结果表明，AFB1的检测线性范围为0．5—50μg／kg，线性相关系数r为0．9997。方法检出限为0．15μg／kg，加标回收率80．1％～109．2％，相对标准偏差（RSD）为0．23％-4．29％。该方法简便、准确、成本低廉，可用于啤酒原辅料中的AFB1的质量控制。