Validation of molecular marker AVRDC-PP12 linked to male sterility gene ms10 of chilli

摘要

The newly identified SSR marker AVRDC-PP12 linked to the male sterility gene ms10 distinguished the heterozygous (Ms10ms10) fertile from the homozygous (Ms10Ms10) fertile plants in chilli segregating populations. To assess efficiency of AVRDC-PP12 in marker-assisted backcrossing for transfer of ms10 gene; the marker was screened in four backcross progenies. The marker co-segregated with ms10 gene in three progenies derived from the crosses 'MS-12 x VR-16', 'MS-12 x S-217621' and 'MS-12 x Selection Dev' with recombination frequency of 3.22, 4.16 and 3.27%, respectively. The marker failed to differentiate the parents in the cross 'MS-12 x DCL-524' and hence was not used for further genotyping of the population.