UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF ROSUVASTATIN CALCIUM AND HYDROCHLORTHIAZIDE IN THEIR COMBINED DOSAGE FORMS

摘要

A simple, accurate, precise, reproducible and economical UV spectroscopic method for simultaneous estimation of Rosuvastatin calcium and Hydrochlorthiazide in marketed formulation has been developed. Method employs solving of simultaneous equations based on the measurement of absorbance at two wavelengths 243 nm and 270 nm which are the Xmax values of rosuvastatin calcium and hydrochlorthiazide in methanol. The linearity of the method was investigated in range of 5-15 ug/mL for rosuvastatin calcium and 6.25-18.75 ug/mL for hydrochlorthiazide, respectively. The results of analyses have been validated statistically for linearity, accuracy and precision, ruggedness, LOD and LOQ of the proposed method. This method can be successfully employed for simultaneous estimation of all drugs in commercial products.