Comparative GC/MS analysis of rose flower and distilled oil volatiles of the oil bearing rose Rosa damascena.

摘要

Improved and successful oil bearing rose breeding requires routine application of an efficient procedure for analysis of flower volatiles, with capacity to extrapolate the obtained data to the volatile composition of distilled rose oil. A procedure for solvent extraction and GC-MS analysis of rose flowers and rose oil volatiles from oil bearing roses, including Rosa damascena, is described. The procedure allows reliable identification of 68 volatiles in rose flowers which are also detected in distilled rose oil. The procedure was used for comparative analysis of flower and distilled rose oil volatiles from 8 different genotypes of oil bearing roses. A data set consisting of ratios of the relative abundance of a given volatile in the flower spectra to the relative abundance of the same volatile in distilled rose oil spectra was generated. ANOVA for a data subset of 27 volatiles detected in flowers and rose oils of all analyzed oil bearing rose genotypes showed no significant influence of genotype on the ratio of relative abundances of flower to rose oil volatiles. The average and relative s.d. of the obtained ratios between relative abundances of flower and rose oil volatiles for genotypes were calculated for each identified flower compound. Results showed that the flower solvent extraction and GC-MS analysis procedure could be used reliably for prediction of the volatile composition of distilled rose oils from a wide range of oil bearing rose genotypes based on the extrapolation of GC-MS analysis data from single or a few flowers from each studied plant. The possibilities for incorporation of the described procedure into oil bearing rose breeding and genetic resource characterization are discussed.