SEQUENCE ANALYSIS OF THE REGIONS FLANKING TERMINAL REPEATS OF THE GENOME OF SUMAVA ISOLATE OF MURINE GAMMAHERPESVIRUS

摘要

The Sumava isolate of murine gamrnaherpesvirus (MHV-Sumava) slightly differs from Murine gammaherpesvirus 68 (MHV-68) and two other isolates of murine gammaherpesvirus (MHV), MHV-76 and MHV-72 in some biological properties. To identify the region(s) in the MHV-Sumava genome responsible for this phenomenon, we compared the sequences flanking terminal repeats (TRs) of the MHV-Sumava genome with those of MHV-68, MHV-76 and MHV-72. Restriction and sequence analyses revealed in MHV-Sumava as compared to MHV-68 a approx9.3 kbp deletion at the left end of the genome and a approx1.5 kbp deletion at the right end of the genome. While the approx9.3 kbp deletion was similar to that in MHV-76, the approx1.5 kbp deletion was unique for MHV-Sumava.