鸭坦布苏病毒江西分离株全基因组序列分析

摘要

为了解江西省鸭坦布苏病毒(Duck Tembusu virus,DTMUV)的遗传变异情况,对2013年江西省某蛋鸭场分离获得的1株鸭坦布苏病毒(命名为YF株)进行基因组分子特征及遗传进化分析.序列分析表明,该株病毒基因组全长10 990 nt,未出现核苷酸插入或缺失及基因重组的变异情况,与已报道的DTMUV分离株的核苷酸同源性均在98.5％以上,且亲缘关系与当前鸭群中优势病毒亚群非常近.以上结果表明,近年来江西鸭群中流行的鸭坦布苏病毒遗传比较稳定,未发生变异.%Genetic characteristics and variation of the epidemic strain of duck Tembusu virus (DTMUV) found in Jiangxi Province were investigated.A DTMUV strain,designated as YF,was isolated from a clinical case in 2013.A sequence analysis showed the viral genome of YF to be 10,990 nt in length with no Ins/Del or genetic recombination.It shared no less than 98.5％ homology with the viral strains isolated from ducks since 2010.Phylogenetically,YF had a close relationship with currently circulating dominant virus lineage.It appeared that the DTMUV in local ducks had been genetically stable throughout these years.