A tissue-implant reaction associated with subcutan implantation of alpha-tricalcium phosphate, dental ceramic and hydroxyapatite bioceramics in rats.

摘要

Calcium phosphate ceramics are among the more commonly used and biocompatible ceramics. Recently, we have synthesized a new calcium phosphate ceramic, alpha tricalcium phosphate (aTCP). The aim of this study was to assess the biocompatibility of this original, in our laboratory modified, newly synthesized aTCP ceramic, by carefully evaluating the inflammatory reaction of soft tissue in response to its subcutaneous implantation and by comparing this result with the results of already widely used virtually non-toxic, non-immunogenic and almost chemically inert dental (DC) and hydroxyapatite (HAP) ceramics. Implants (diameter 5x2 mm) of aTCP, DC and HAP were implanted into 12 adult male rats subcutaneously. At 2 and 12 weeks post-implantation, the animals were euthanized and the tissue-implant reactions were analysed histologically. Evaluation of routine stained sections (5 micro m, haematoxylin and eosin) of the cutis and subcutis surrounding the aTCP, DC and HAP ceramics revealed the following: (1) all the ceramic devices had fibrous connective tissue capsules; (2) there were significant differences in the tissue-implant reactions based on the estimated time, while there were no differences in the tissue-implant reaction based on the type of ceramic material and (3) two weeks after implantation hyperemia and cellular proliferation were the most expressive results, while twelve weeks after implantation extensive angiogenesis and collagen fibres production were the prominent findings for all types of implanted calcium phosphate ceramics.