A novel method for sensitive detection ofEscherichia coliO157:H7 based on an aptamer and hybridization chain reaction

摘要

This work developed a novel assay for the detection ofEscherichia coliO157:H7 (E. coliO157:H7), which is based on the specific recognition of an aptamer (H0) forE. coliO157:H7, the signal amplification of a hybridization chain reaction (HCR) and the adsorption and quenching effect of graphene oxide (GO). The feasibility of this work was verified through control experiments. The concentration of GO, the HCR time, the concentration of H0, and the concentration of H1/H2 were optimized. A series ofE. coliO157:H7 dilutions were detected under the optimal conditions, and the detection range was 2.6 x 10(2)to 2.6 x 10(8)CFU mL(-1). The linear equation isy= 591.1x- 943.9,R-2= 0.995, and LOD is 5 x 10(1)CFU mL(-1). Milk samples were used for the spike recovery experiment, and the recovery rate was 92.6-103.6%. This method shortens the detection time greatly and provides a new strategy for food safety monitoring.