Construction and Studies of Histamine Potentiometric Sensors Based on Molecularly Imprinted Polymer | Bentham Science

摘要

Objective: Molecularly Imprinted Polymer (MIP)-modified potentiometric sensors for histamine(HIS) (as denoted as HIS sensor) have been developed.Methods: The MIPs comprise HIS, Methacrylic Acid (MAA) and ethylene glycol dimethacrylate asthe template molecule, functional monomer and cross-linker, respectively. To examine the specificityof the MIP to HIS, the MIP particles were prepared with varying ratios of HIS: MAA and the HISbinding amount toward the MIP particles was determined by UV spectrophotometry. Furthermore, toquantitatively determine the ability of MIP (H2M20) to HIS, a HIS sensor was measured usingAg/AgCl as a reference electrode.Results: MIP particles having a HIS:MAA of 2 mmol:20 mmol (MIP (H2M20)) had the largest HISbinding amount among the MIP particles prepared. Additionally, MIP (H2M20) displayed a HISbinding amount approximately two times larger than the corresponding non-imprinted polymer (NIP)particles in the absence of template. The HIS sensor potential change increased as a function of HISconcentration and exhibited a near-Nernstian response of ?25.7 mV decade?1 over the HIS concentrationrange of 1×10?5 to 1×10?4 mol L?1 with a limit of detection of 9.6×10?6 mol L?1. From theNernstian response value, it was observed that the HIS sensor could detect the di-protonated HISbinding to the MIP. Conversely, when comparing at the same HIS concentration, the potential responsevalue of the sensors fabricated using NIP particles were significantly smaller than the valuesof the corresponding HIS sensor.Conclusion: The MIP-modified potentiometric sensors can potentially be employed as an analyticalmethod to quantitatively determine various analytes.