Effects of Improved DNA Integrity by Punch From Tissue Blocks as Compared to Pinpoint Extraction From Unstained Slides on Next-Generation Sequencing Quality Metrics

Morlote Diana; Janowski Karen M.; Siniard Rance C.; Guo Rong Jun; Winokur Thomas; DeFrank Gina; Harada Shuko

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Effects of Improved DNA Integrity by Punch From Tissue Blocks as Compared to Pinpoint Extraction From Unstained Slides on Next-Generation Sequencing Quality Metrics

机译：与组织块的改善DNA完整性的影响与在下一代测序质量指标上的未染色载玻片的针刺提取

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Objectives To compare the effects of two methods of formalin-fixed paraffin-embedded (FFPE) tissue harvesting on DNA quality and next-generation sequencing (NGS) quality metrics.

机译：目标，比较两种福尔马林固定石蜡包埋（FFPE）组织收获对DNA质量和下一代测序（NGS）质量指标的影响。

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来源
《American journal of clinical pathology.》 |2019年第1期|共9页
作者
Morlote Diana; Janowski Karen M.; Siniard Rance C.; Guo Rong Jun; Winokur Thomas; DeFrank Gina; Harada Shuko;
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作者单位

Univ Alabama Birmingham Birmingham AL 35294 USA;

Univ Alabama Birmingham Birmingham AL 35294 USA;

Univ Alabama Birmingham Birmingham AL 35294 USA;

Univ Alabama Birmingham Birmingham AL 35294 USA;

Univ Alabama Birmingham Birmingham AL 35294 USA;

Univ Alabama Birmingham Birmingham AL 35294 USA;

Univ Alabama Birmingham Birmingham AL 35294 USA;

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原文格式 PDF
正文语种 eng
中图分类病理学;
关键词
Next-generation sequencing; Tissue harvesting; Formalin-fixed paraffin-embedded; DNA integrity number; Quality metrics; Preanalytical;

机译：下一代测序;组织收获;福尔马林固定石蜡嵌入;DNA完整性数量;质量指标;PREENALYTICAL;

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专利

1. Effects of Improved DNA Integrity by Punch From Tissue Blocks as Compared to Pinpoint Extraction From Unstained Slides on Next-Generation Sequencing Quality Metrics [J] . Morlote Diana, Janowski Karen M., Siniard Rance C., American journal of clinical pathology. . 2019,第1期

机译：与组织块的改善DNA完整性的影响与在下一代测序质量指标上的未染色载玻片的针刺提取
2. Improving DNA quality from formalin-fixed paraffin-embedded tissue extraction for next-generation sequencing applications [J] . Iley N., Davison V., Deans Z., Virchows Archiv: an international journal of pathology . 2017,第Suppla1期

机译：从福尔马林固定的石蜡包埋的组织提取改善下一代测序应用的DNA质量
3. Rapid Loss of RNA Detection by In Situ Hybridization in Stored Tissue Blocks and Preservation by Cold Storage of Unstained Slides [J] . Baena-Del Valle Javier A., Zheng Qizhi, Hicks Jessica L., American journal of clinical pathology. . 2017,第5期

机译：通过原位杂交在储存的组织块中的快速丧失RNA检测，并通过未染色的载玻片的冷储存
4. On Comparing Software Quality Metrics of Traditional vs Blockchain-Oriented Software: An Empirical Study [C] . Marco Ortu, Matteo Orrú, Giuseppe Destefanis IEEE International Workshop on Blockchain Oriented Software Engineering . 2019

机译：传统与面向区块链的软件的软件质量指标比较：一项实证研究
5. The Effects of Commercial Cleaning Agents on Automated DNA Extraction Efficiency and Genetic Profile Quality [D] . Taylor, Allison R. 2015

机译：商业清洁剂对DNA自动提取效率和遗传图谱质量的影响
6. Histopathological factors affecting the extraction of high quality genomic DNA from tissue sections for next-generation sequencing [O] . Satoshi Fujii, Takayuki Yoshino, Kentaro Yamazaki, -1

机译：影响从组织切片中提取高质量基因组DNA进行下一代测序的组织病理学因素
7. Assessment of the quality of DNA from various formalin-fixed paraffin-embedded (FFPE) tissues and the use of this DNA for next-generation sequencing (NGS) with no artifactual mutation. [O] . Naoki Einaga, Akio Yoshida, Hiroko Noda, 2017

机译：评估来自各种福尔马林固定的石蜡包埋（FFpE）组织的DNa质量，以及将该DNa用于下一代测序（NGs），没有人为突变。

Effects of Improved DNA Integrity by Punch From Tissue Blocks as Compared to Pinpoint Extraction From Unstained Slides on Next-Generation Sequencing Quality Metrics

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