Potential role of Kisspeptin and Cytochrome P mRNA genes in pubertal development and spermatogenesis in buffalo bulls (Bubalus bubalis)

摘要

Puberty and spermatogenic activity are associated with higher expression of hypothalamic Kisspeptin gene and steroidogenic enzyme gene and may be useful in understanding the biological regulation of puberty in buffalo bulls.Kisspeptin, a neuropeptide produced by the KiSS1 gene, stimulates gonadotropin releasing hormone (GnRH) which is required for puberty. Increased KiSS1r mRNA expression has been observed at the time of puberty in rat, cattle, goat, mouse, monkey (d'Anglemont de Tassigny X and Colledge, 2010). Despite a few reports on the distribution of KiSS1 gene in various regions of hypothalamus in dairy buffaloes, the expression of KiSS1 gene in the hypothalamus of buffalo bulls is lacking. Therefore, we aimed at examining the expression of KiSS1 mRNA in different areas of hypothalamus and cytochrome P450 side-chain cleavage, P450scc (CYP11A1), a rate limiting enzyme of steroidogenesis, in the testicular tissues and their correlations with various determinants of gonadal histomorphometry in buffalo bulls.