Comparison of Ramping Speeds of the Micro PCR Chip Reaction Chamber

摘要

In this study, an analysis of the correlation between the reagent ramping speeds of micro polymerase chain reaction (PCR) chips based on printed circuit boards and the thickness of the reaction chamber was described. The micro PCR chip presented in this study is composed of a heating-patternand a thermistor. The former is used to heat the reagent and the latter is used to measure the temperature on the opposite side of the chamber. In addition, reagent temperature was measured by inserting a thin thermocouple wire inside the chamber. The correction coefficient of the reagenttemperature that was measured to be lower than the thermistor temperature is found by using the temperature measured by the thermocouple that is the actual reagent temperature and the ramping speeds by chamber thickness of the micro PCR chip are compared. Results showed that the ramping speedof the thermocouple temperature measured in the 200-μm-thick chamber was approximately 1 sec faster than that obtained with the 400-μm-thick chamber. Furthermore, it was found that the overall ramping speed increases in thinner chambers.