Fluorescent Histochemical Localization of Dipeptidyl Peptidase IV Activity

摘要

A novel fluorogenic substrate for dipeptidyl peptidase IV (DPP IV) - Ala-Pro-4-hydrazido-N-(4'-isobutoxy)pheny1-1,8-naphthalimide (Ala-Pro-BuOPHNI) and its application for the localization of the enzyme in tissue sections of rat organs are presented. The substrate permits a precise detection of DPP IV activity in situ and might find various applications in the studies of the diseases for which DPP IV is a marker enzyme.