New Synthetic Fluorescent Substrate for Histochemical Localization of the Enzyme Dipeptidyl Peptidase II on the Base of 1,8-Naphthalimides

摘要

A novel fluorescent substrate L-Lys-L-Ala-6-hydrazido-N-hexy1-1,8-naphthalimide for the histochemical visualization of activity distribution of Dipeptidyl peptidase II is proposed. By now, a fluorescent determination of the DPP II activity is realized only in cell homogenates and cultivated cells, whereas fluorescent method for the enzyme localization in tissue sections is missing. The results of our work show that the new method permits precise localization and visualization of dipeptidyl peptidase II in tissue sections from several Wistar rat organs.