Effect of Seasonal and Regional Variations on Phenolic Compounds of Deverra scoparia (Flowers/Seeds) Methanolic Extract and the Evaluation of Its in Vitro Antioxidant Activity

摘要

The widespread use of Deverra scoparia COSS. & DURIEU in Algerian folk-medicine as a remedy can be relatively attributed to its total phenolic compounds. The current study aimed to provide a scientific basis for optimal collection and usage of Deverra scoparia COSS. & DURIEU plant. Hence, 37 samples were gathered from nine sites in Algeria during two seasons 2016 and 2017, then exposed to a green extraction. Total phenolic (TPC), flavonoid (FC) and condensed tannins (CTC) content were estimated spectrophotometrically. The antioxidant activity was measured using five different methods, DPPH~·, ABTS~(·+), FRAP, CUPRAC and Fe~(2+)-chelating. The results have revealed considerable amounts of TPC varied from 804 to 1544 mg GAE/100 g dry matter, FC started from 187 up to 410 mg QE/100 g dry matter and CTC varied from 111 to 394 mg CE/100 g dry matter. The best IC_(50) values (μg/mL) of DPPH~·, ABTS~(·+), FRAP, CUPRAC and Fe~(2+)-chelating tests were 56.62, 5.41, 21.26, 52.93 and 78.10, respectively. Moreover, high correlations were found between CTC and most of the antioxidant tests. Hence, CTC are suggested to be the principal group of antioxidant activity in Deverra scoparia COSS. & DURIEU extracts.