首页> 外文期刊>Bulgarian Journal of Agricultural Science >Genotype frequencies in calpastatin (CAST) and callipyge (CLPG) genes in Northeast Bulgarian Merino sheep breed using PCR-RFLP method
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Genotype frequencies in calpastatin (CAST) and callipyge (CLPG) genes in Northeast Bulgarian Merino sheep breed using PCR-RFLP method

机译:利用PCR-RFLP方法,钙钾素(铸造)和CALLIPYGE(CLPG)基因的基因型频率和CALLIPYGE(CLPG)基因

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摘要

The purpose of the present work was to identify polymorphic allelic variants of ovine calpastatin and callipyge genes in Northeast Bulgarian Merino sheep breed. Blood samples were collected from 32 rams, reared in Agricultural Experimental Station - Targovishte. Genomic DNA was extracted using commercial kit for DNA purification from whole blood. After PCR amplification of CAST gene with specific primer set a fragment of 622 bp was obtained. CLPG gene also was amplified with specific primers and a fragment of 426 bp was obtained. Genotypes were determined by restriction fragment length polymorphism (RFLP) method with MspI restriction enzyme for CAST gene and BsmFl restriction enzyme for CLPG gene. Calpastatin locus was found to be polymorphic, whereas in callipyge gene it was observed uniformity - the presence of genotype AA only. Genotype frequencies established in CAST gene were 0.47% and 0.53% for MM and MN, respectively, whereas genotype NN was not found.
机译:本作工作的目的是鉴定东北保加利亚美奴羊繁殖的羊钙素和Callipyge基因的多态等位基因变体。 从32例RAM中收集血液样品,饲养在农业实验站 - Targovishte。 使用商业试剂盒提取基因组DNA,用于从全血中进行DNA纯化。 在具有特异性引物的铸基的PCR扩增后,获得622bp的片段。 CLPG基因也用特异性引物扩增,得到426bp的片段。 基因型通过限制性片段长度多态性(RFLP)法测定具有MSPI限制酶的用于CLPG基因的MSPI限制酶和BSMFL限制酶。 发现钙帕喇草基因座是多态的,而在Callipyge基因中,观察到均匀性 - 仅存在基因型AA。 在铸基中建立的基因型频率分别为mm和mn的0.47%和0.53%,而没有发现基因型Nn。

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