Analysis of Polymorphism of Caplstatin and Callipyge Genes in Saudi Sheep Breeds Using PCR-RFLP Technique

摘要

The first part of the present work dealt with the extraction and assay of lectin obtained from white truffles Terfezia Bouderei. The major technique employed was chromatography on dextran gel G-75, the activity of lectin is inhibited by mannose, each preparation was checked of lectin activity, the later was measured by the amount of agglutination produced using a buffered and stabilized suspension (3%) rabbit red blood cells. The second part of the study included the immuno-stimulation activity of phagocytes. Immunomodulatory activity of extracted lectins from Terfezia Bouderei was evaluated on phagocytic activity by carbon clearance test. Adult Albinos Wistar mice randomly divided into four groups, were the first was served as a control, while the remaining groups respectively treated with extracted lectins from Terfezia Bouderei at dose of: 10, 30 and 50 mg/kg by intra-peritoneal injection (IP). Change in phagocytic activity was determined after 48 h injection of carbon ink suspension. In carbone clearance test, extracted lectins from Terfezia Bouderei exhibited significantly phagocytic index dose-dependent against control group, indicating stimulation of the reticulo-endothelial system. Present study thus reveals that extracted lectins from Terfezia Bouderei holds promise as immunomodulatory agent, which act by stimulating dose dependent phagocytic function.