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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Validation of an analytical method to quantify the permeation and penetration of flurbiprofen into human pharynx tissue
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Validation of an analytical method to quantify the permeation and penetration of flurbiprofen into human pharynx tissue

机译:验证分析方法,以量化氟氯芬渗透和渗透到人咽组织中的渗透和渗透

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摘要

Abstract The aim of this investigation was to develop receiver and extraction fluids, and subsequently validate an analytical method to quantify the permeation and penetration of flurbiprofen into human pharynx tissue using a Franz diffusion cell. The solubility and stability of flurbiprofen in a suitable receiver fluid, and a suitable extraction method and fluid to recover and quantitate flurbiprofen from human pharynx tissue, were investigated using high‐performance liquid chromatography (HPLC). The potential interference of human pharynx tissue in the receiver fluid was also investigated. The HPLC analytical method was successfully validated according to current guidelines. The final receiver fluid demonstrated sufficient solubility and stability, and the extraction method and fluid resulted in 95% recovery of flurbiprofen following exposure to human pharynx tissue. The lower limit of quantitation of flurbiprofen was 0.045?μg/mL in both the receiver and extraction fluids. There was no interference of the human pharynx tissue with the HPLC method. This investigation validated an analytical method for quantitating flurbiprofen, and determined a suitable receiver fluid and extraction method and fluid, which can be used to investigate the permeation and penetration of flurbiprofen through human pharynx tissue using the Franz diffusion cell method.
机译:摘要该研究的目的是开发接收器和提取流体,随后验证使用FRANZ扩散细胞来定量渗透和渗透到人咽组织中的分析方法。使用高效液相色谱(HPLC)研究了合适的接收器流体中Flbiprofof的溶解度和稳定性,以及从人咽组织中回收和定量Flbiprofen的合适的提取方法和流体。还研究了人咽部组织在接收器流体中的潜在干扰。根据当前指南成功验证了HPLC分析方法。最终的接收器流体表现出足够的溶解度和稳定性,并且提取方法和流体导致&暴露于人咽组织后的氟泡芬的95%回收。在接收器和萃取流体中,Flbriprofof的定量下限为0.045Ωμg/ ml。人咽组织与HPLC方法没有干扰。该研究验证了用于定量氟菊酯的分析方法,并确定合适的接收器流体和提取方法和流体,其可用于使用FRANZ扩散细胞方法研究流动通过人咽组织的渗透和渗透。

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