Gold Immunochromatography Assay for the Rapid Detection of Spiramycin in Milk and Beef Samples Based on a Monoclonal Antibody

机译：基于单克隆抗体的牛奶和牛肉样品快速检测的金免疫层析测定

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Abstract > Spiramycin (SP) residues in food do harm to human health. It is necessary to establish rapid detection method for SP. In this work, a monoclonal antibody (mAb)‐based gold immunochromatography assay (GICA) is developed for the rapid detection of SP. Under optimum conditions, the half‐maximal inhibitory concentration of SP‐mAb is 0.43?ng?mL –1 . The subtype of SP‐mAb is IgG2b. This antibody has no cross‐reactivity with other analogues and has high affinity (4.52 × 10 10 L?mol –1 ). Qualitative results can be visualized with the naked eye, with a visual detection limit of 1.0?ng?mL –1 and cut‐off value of 10?ng?mL –1 . A hand‐held strip scanner is used for the quantitative analysis, with LOD 0.43?ng?mL –1 in assay buffer. The recoveries of SP ranged from 72.3% to 112% in milk and 98.5% to 115% in beef, with variable coefficient ranging from 9.4% to 11.7% in milk and 8.14% to 15.4% in beef. Besides, the proposed GICA method for SP is confirmed by LC–MS/MS in SP‐spiked milk and beef samples. Overall, the developed GICA can be a useful tool for SP residues on‐site screening in milk and beef samples. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 斯皮霉素（SP）食物残留物对人体健康造成伤害。有必要建立SP的快速检测方法。在这项工作中，开发了一种单克隆抗体（MAB）基于Gold免疫层析测定（GICA），用于快速检测SP。在最佳条件下，Sp-mab的半最大抑制浓度为0.43Ω·ng？ml -1 </ sup> 。 SP-MAb的亚型是IgG2B。该抗体与其他类似物没有交叉反应性，具有高亲和力（4.52×10 10 </ sup> l？mol. -1 </ sup> ）。定性结果可以用肉眼可视化，视觉检测限为1.0？ng？ml -1 </ sup> 和截止值10？ng？ml -1 </ sup> 。手持条带扫描仪用于定量分析，LOD 0.43？NG？ML -1 </ sup> 在测定缓冲液中。 SP的回收率从牛奶中的72.3％至112％的速度范围为72.3％至112％，牛肉中的98.5％至115％，变量系数为9.4％至11.7％，牛肉中的8.14％至15.4％。此外，在SP掺入牛奶和牛肉样品中，LC-MS / MS确认了所提出的GICA方法。总的来说，发达的GICA可以是SP残留在牛奶和牛肉样品中的SP残留物的有用工具。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15025/'>《Biotechnology Journal: Healthcare,Nutrition,Technology》</a> <b style="margin: 0 2px;">|</b><span>2020年第1期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guo Lingling&option=202" target="_blank" rel="nofollow">Guo Lingling;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Liqiang&option=202" target="_blank" rel="nofollow">Liu Liqiang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xu Liguang&option=202" target="_blank" rel="nofollow">Xu Liguang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kuang Hua&option=202" target="_blank" rel="nofollow">Kuang Hua;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cui Gang&option=202" target="_blank" rel="nofollow">Cui Gang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xu Chuanlai&option=202" target="_blank" rel="nofollow">Xu Chuanlai;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi 214122 P. R. China;</p> <p>State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi 214122 P. R. China;</p> <p>State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi 214122 P. R. China;</p> <p>State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi 214122 P. R. China;</p> <p>Department of BiotechnologyYancheng Teachers UniversityYancheng 22400 P. R. China;</p> <p>State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi 214122 P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=beef&option=203" rel="nofollow">beef;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gold immunochromatography assay&option=203" rel="nofollow">gold immunochromatography assay;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=milk&option=203" rel="nofollow">milk;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=spiramycin&option=203" rel="nofollow">spiramycin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=strip&option=203" rel="nofollow">strip;</a> </p> <div class="translation"> 机译：牛肉;金免疫层析测定;牛奶;螺霉素;带; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021882744.html">Gold Immunochromatography Assay for the Rapid Detection of Spiramycin in Milk and Beef Samples Based on a Monoclonal Antibody</a> <b>[J]</b> . <span> <a 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