Technology of fermentation coupling with foam separation for improving the production of nisin using a kappa-carrageenan with loofa sponges matrix and an hourglass-shaped column

摘要

There are two problems in the technology of the fermentation coupling with foam separation: stripping of bacteria into the foam and large volume of interstitial liquid. In order to solve these problems, cell immobilization was utilized in this technology and a new carrier by combining kappa-carrageenan and loofa sponge was designed to successfully immobilize Lactococcus lactis supsp. Lactis ATCC 11454. A novel hourglass-shaped column was developed to effectively enhance foam drainage. Continuous fermentation of immobilized cells coupling with foam separation was carried out for 100 h and a maximum total nisin titer of 4.91 x 10(3) IU/mL was achieved. Nisin productivity of 3.92 x 10(2) IU/mLh in continuous + coupled foam separation was higher than that in continuous + uncoupled foam separation (3.20 x 10(2) IU/mL h) and batch fermentation (2.36 x 10(2) IU/mL h). The current study demonstrated that this technology significantly enhanced the production of nisin and would be useful in scaling-up of the fermentation process for improving the production of nisin. (C) 2018 Elsevier B.V. All rights reserved.