An edible fungi Pleurotus ostreatus inhibits adipogenesis via suppressing expression of PPAR gamma and C/EBP alpha in 3T3-L1 cells: In vitro validation of gene knock out of RNAs in PPAR gamma using CRISPR spcas9

摘要

Objective: Obesity is now well recognized as a disorder, one that is essentially preventable through changes in lifestyle. Obesity is also a main concern associated with expanded morbidity and mortality from many noncommunicable illnesses (NCDs). The study aimed to determine the antiobesity effect of Pleurotus ostreatus (PO) and its bioactive anthraquinone (AQ). The overall promoter genes CEBP alpha (CCAAT enhancer binding protein alpha) and PPAR gamma (Peroxisome proliferator activated receptor gamma) in controlling the homeostasis of glucose was analysed using 3T3-L1 cell line. Finally, an insilico study was carried out using CRISPR software to identify the RNA's involved in adipogenesis especially of the control gene PPAR gamma.