HPLC-MS Analysis of Sennosides A and B in Aqueous Extracts of Sennae folium Prepared by a New Microwave Extraction Method

摘要

A new, simplified open-vessel microwave extraction (OVME) method with solid-phase extraction (SPE) for sample clean-up has been developed for specific investigation of sennosides A and B. Aqueous extracts of senna leaves (Senna folium) were prepared by different methods, including classic pharmacopoeial methods: concoction, infusion, and ultrasound extractions. The different extraction methods were compared by analysis of the sennosides A and B in the samples with a modified enantiomer-selective reversed-phase high-performance liquid chromatographic-tandem mass spectrometric method. HPLC was performed with a 4.6 mm x 150 mm, 3.5-mu m particle C-8 column and simple gradient elution. The benefit of open-vessel microwave extraction is discussed.