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Study on a relation between estrogen receptor (ESR) gene polymorphism and some pig reproduction performance characters in Polish Landrace breed

机译:波兰地方品种雌激素受体(ESR)基因多态性与部分猪繁殖性能关系的研究

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The study was carried out in a pedigree herd of 8 boars and 207 sows of the Polish Landrace breed. The polymorphism of the ESR gene was found by means of the PCR-RFLP method. The amplification resulted in a product of 120 base pairs. The product was then digested with the PvuII restriction enzyme. The two alleles of the ESR gene were identified - ESRA allele - 120 bp, ESRB - 65 and 55 bp. The frequency of ESRA was 0.9419, whereas that of ESRB was 0.0581. The frequency of the AA genotype was 0.8837, whereas that of AB was 0.1163. The ESR BB genotype was not found in the pig population under study. The study focused on the relation between ESR/PvuII genotypes and the number of teats on the left and on the right side, total number of teats in sows, number of piglets in the 1st, 2nd, 3rd, 4th and 5th fitters and farrowing age of the first five fitters. The initial data for calculations were transformed from the discrete scale to the normal one. The analysis of the relation between the ESR genotypes and reproduction performance characters in the pedigree pigs in five subsequent fitters showed small and statistically insignificant differences between the sows of ESR AA and ESR AB genotypes.
机译:这项研究是在8个公猪和207个波兰地方品种的母猪的系谱中进行的。通过PCR-RFLP方法发现了ESR基因的多态性。扩增产生120个碱基对的产物。然后将产物用PvuII限制酶消化。鉴定出ESR基因的两个等位基因-ESRA等位基因-120 bp,ESRB-65和55 bp。 ESRA的频率为0.9419,而ESRB的频率为0.0581。 AA基因型的频率为0.8837,而AB基因型的频率为0.1163。在研究的猪群中未发现ESR BB基因型。该研究集中在ESR / PvuII基因型与左右奶头数量,母猪奶头总数,第1、2、3、4和5仔猪仔猪数量和分娩年龄之间的关系上。前五个钳工中的一个。用于计算的初始数据已从离散比例转换为正常比例。在五只随后的钳工中,对系谱猪的ESR基因型与繁殖性能特征之间的关系进行分析后,发现ESR AA和ESR AB基因型母猪之间的差异很小,但在统计学上无统计学意义。

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