The Family of Serratia Type Pore Forming Toxins

摘要

The Serratia marcescens hemolysin represents the prototype of a growing family of pore forming toxins.The available bacterial genome sequences reveal Serratia hemolysin.,homologues in additional species.However,only S.marcescens hemolysin has been studied in great molecular detail.This family of toxins has nothing in common with the pore forming toxins of E.coli type (RTX toxins),the Staphylococcus aureus cc-toxin or the thiol activated toxin of group A beta-hemolytic streptococci (Streptolysin O).Studies on erythrocytes,eukaryotic cells and artificial black lipid membranes,have shown that the mechanism of pore formation of ShlA is different form other pore forming toxins.The S.marcescens hemolysin proteins ShlB and ShlA,exhibit protein sequence homologues in Proteus mirabilis,Haemophilus ducreyi,Yersinia pestis,Yersinia enterocolitica,Edwardsiella tarda,Pho-torhabdus luminescens and Xylella fastidiosa .The family of Serratia type pore forming toxins show a unique secretory mechanism which has been described as a two partner secretion system (TPSS) or type V-secretion system.Not only Serratia type pore forming toxins are secreted via TPSS but also adhesins from Bordetella pertussis,Er-winia chrysanthemi and Haemophilus influenzae.The uniqueness of the Serratia family is underlined by the fact that activation of ShlA by ShlB strictly requires phosphatidylethanolamine as a cofactor.And,quite unusual,ShlA undergoes a conformational change during activation.