Validated LC-MS/MS method for simultaneous quantification of seven components of Naodesheng in rat serum after oral administration and its application to a pharmacokinetic study

摘要

A simple, precise and reliable LC-MS/MS method was developed and validated for simultaneous quantification of vitexin, notoginsenoside R1, hydroxysaffior yellow A, ginsenoside Rd, puerarin, daidzein and senkyunolide I as components of Naodesheng (NDS) in rat serum. The Linearity ranges in rat serum were 0.045-4.5 mu g/mL for vitexin, 0.0476-4,76 mu g/mL for notoginsenoside R1, 0.0422-4.22 mu g/mL for hydroxysaffior yellow A, 0.0426-4.26 mu g/mL for ginsenoside Rd, 0.0436-4.36 mu g/mL for puerarin, 0.026-2.6 mu g/mL for daidzein, and 0.05-5 mu g/mL for senkyunolide I, with the correlation coefficients greater than 0.99. The established method was validated in terms of intra- and inter-day precision and accuracy, recovery, matrix effect and stability. Furthermore, the method was successfully applied for pharmacokinetic study of these seven components in rat serum after oral administration of NDS. (C) 2019 Elsevier B.V. All rights reserved.