An effective method for the determination of the enantio-purity of L-alpha-glycerophosphocholine (L-alpha-GPC)

摘要

Herein we describe for the first time a practical analytical method for determining the enantio-purity of L-alpha-glycerophosphocholine (L-alpha-GPC) by chiral derivatization. The use of a suitable chiral boronic acid allowed the formation of a mixture of two diastereomeric boronate esters whose ratio, carefully analyzed by iHNMR spectroscopy, reflects L-alpha-GPC enantiomeric excesses. The determination of the enantiopurity of L-alpha-GPC furnishes convincing correlations with its manufacturing process. (C) 2020 Elsevier B.V. All rights reserved.