Isolation of E. coli Ol57:H7 Bacteriophages of Livestock Waste of Mazandaran Province (Case Study: A Phage Therapy Approach)

摘要

Phage therapy for E .coli 0157:H7 in animals is considered as a popular research topic. E .coli Ol57: H7 is a pathogenic potential. It can cause hemolytic uremic syndrome (HUS). Considering this matter, the present research aims at analyzing the isolation of E. coli 0157:H7 bacteriophages of livestock waste of Mazandaran province using a phage therapy approach. The separation and culture processes of E .coli Ol57:H7 from livestock wastewater have been analyzed in this research. Also, differential tests were used to identify E. coli 0157:H7. First, the Lytic bacteriophages of E .coli 0157:H7 were isolated from livestock wastewater. Then, both identification and susceptibility tests for bacteria E. coli Ol57:H7 were done and phages were isolated usingvarious methods. Finally, phage therapy to animal model was conducted. In this study, I have used two methods including disk diffusion and overlay tests. This test can be used for three strains standards including Enteropathogenic E. coli (EPEC), Enterotoxigenic E.coli (ETEC), Enteroinvasive E. coli (EIEC), and wild E. coli Ol57:H7. According to the results, 45 samples had lytic phages vs. four different E .co/istrains and only 19 samples had significant effect on E. coli 0157: H7. Also, it can be concluded that isolated phages affect bacteria in a significant manner (p<0.05). In the current research, the isolated lytic phages removed the infection of E.coli 0157: H7 of five groups of mice. As a result, the lytic bacteriophages have the ability to replace antibiotics.