Preparation and characterization of papain embedded in magnetic cellulose hydrogels prepared from tea residue

摘要

Papain was embedded and immobilized in hydrogels prepared from tea cellulose by dissolving in ionic liquid of 1-ally)-3-methylimidazolium chloride and coating with magnetic Fe3O4 via reaction of FeCl3/FeCl2 solution with ammonium hydroxide. The prepared magnetic-cellulose-hydrogel-embedded papain was characterized via vibrating sample magnetometer, scanning electron microscope, X-ray diffraction, Fourier transform infrared, thermogravimetry analysis and differential scanning calorimetry. Thermal stability, optimal pH, optimal temperature, Michaelis constant and maximum reaction rate were compared between free papain and magnetic-cellulose-hydrogel-embedded papain. The magnetic-cellulose-hydrogel-embedded papain was sensitive to magnetic field and showed paramagnetic behavior, higher thermal stability and lowed substrate affinity. The optimal pH and optimal temperature of the magnetic-cellulose-hydrogel-embedded papain were shifted to 8.0 and 90 degrees C respectively. (C) 2017 Elsevier B.V. All rights reserved.