Molecular Identification of Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli Isolated From Dairy Cows in East Java Province, Indonesia

摘要

Occurence of extended spectrum beta-lacta-mases (ESBL) in Escherichia coli (E. coli) from animal origin is a growing health concern of global significance. The objective of this study was to determine the occurrence 6f ESBL producing E. coli and the characteristics of their encoding genes from 115 rectal swab samples of dairy cows from Tululungagung and Surabaya farms. All samples were positive for Escherica coli as per indole test. To confirm the ESBL the Double Disc Synergy Test (DDST). Betalactamantibiotic disk namely Amoxicylyn-clavulanate, Ceftazidime and Cefotaxime were used for DDST. Molecular identification for bla_(CTXM) and bla_(TEM) ESBL encoding genes was done by used Polymerase Chain Reaction. The Escherichia coli bacteria isolated from rectal swabs of dairy cows was 5.21% (6/115). PCR results showed that bla_(CTX_M) gene was 6 ESBL isolates and bla_(TEM) gene was 2 ESBL isolates. It can be concluded that dairy cows can be potential as reservoir for spreading ESBL isolates to human health.