OPTIMAL PH CONDITIONS FOR THE GROWTH, RELEASE AND STABILITY OF JAPANESE ENCEPHALITIS VIRUS IN PS CELL LINE

摘要

Effects of pH of the medium on the growth, release and stability of Japanese encephalitis virus (JEV) were studied with an aim to optimize the conditions for the virus stock preparation, Eagle's minimum essential medium (MEM) was modified by addition of 30 mM HEPES and reduction of NaHCO3 to 0.85 g/l. The pH values were adjusted to 7.2, 7.4, 7.6, 7.8 and 8.0 by 1.0 N HCl or 1.0 N NaOH. The study revealed that the optimum pH values for the growth, release and stability of JEV were 7.4, 7.8 and 7.6 respectively. [References: 11]