Identification of new genes in human chromosome 3 contig 7 by graphical representation technique

摘要

The rapidly growing library of genomic length sequences and the working draft of the human genome sequence imply a concomitant need to determine new methods to analyse the sequences for rapid identification of new genes and their functions. We have developed a graphical technique for quick determination of probable coding regions in DNA sequences. In this article we apply this technique to the new sequence data from human chromosome 3 contig 7 to test the efficacy of the proposed system in a live case, and also compare with results from other genomic sequences. We report here a sampling of sequence segments that pass theoretical tests of likelihood of being genes and list several that have close homology with sequences from the expressed sequence tag (EST) databases. We also comment on the possible use of the graphical representation technique in the shotgun method of gene sequencing.