芥菜型油菜B03染色体黄籽基因区域BAC重叠群的构建及分析

摘要

The yellow seed genes were located in 1. 5 cM region of B03 linkage group of Brassica juncea in previous stud-ies. Yellow seed genetic regions (through the mustard RNASeq technology of rape seed non redundant genes)and BESs tightly linked to yellow seed genes of B03 were used to design markers in this study,and the markers were used to screen Brassica juncea mapping population parents purple leaf mustard BAC library (ZBjuH BAC library). In this study,320 markers were exploited and 920 BACs screened out from them,483 BACs of which were subject to end sequencing,and 860 sequences were returned. Six contigs (about 3. 5 Mb)were constructed around yellow seed coat gene on B03 chromosome in B. juncea. It was found that highly repetitive sequences are present in the area with yellow seed B03,A03,and A09.%在前人研究中，芥菜型黄籽基因被定位到B03连锁群的1.5 cM区域内。本试验利用与B03染色体控制黄籽基因区域紧密连锁的unigene（通过RNA＿seq技术获得的芥菜型油菜种皮的非冗余基因）和BESs开发标记，并对芥菜型油菜作图群体亲本紫叶芥BAC文库（ZBjuH BAC文库）进行筛选，由开发的320对引物共筛到BAC 920个，对其中483个BACs进行了末端测序，返回序列860条。构建了6段芥菜型油菜B03染色体黄籽区域BAC重叠群，共计长约3.3 Mb；通过BES blast分析表明，在B03染色体黄籽区域与A03、A09染色体存在高度的重复序列。