A modified method to isolate genomic DNA from plants without liquid nitrogen.

摘要

This paper presents a quick, simple and cheap procedure to isolate DNA from plants, which involves alternate cold (-80 degrees C) and heat shock (60 degrees C) treatments in order to break down the cell wall without using liquid N₂. Samples of young, tender, leaves of some plants species (including Desmodium giganticum, Aegle marmelos, Solanum xanthocarpum, Solanum indicum, Tribulus terrestris, Oroxylum indicum, Boerhavia diffusa, Trianthema portulacastrum, Trianthema monogyna and Datura innoxia [D. metel]), collected from the Botanical Garden of Panjab University, Chandigarh, and various other nurseries in India, were used. In conclusion, this method can be used for extraction of DNA of high-quality and high yield from any type of plant tissue and is suitable for all types of molecular biology experiments. The extracted DNA was stable and gave the same results in PCR, microsatellite fingerprinting, PCR-restriction fragment length polymorphism and random amplified polymorphic DNA after 2 years of storage at 4 degrees C.