Mitogenic and melanogenic activity of human placental protein/peptides on melanoma cell

摘要

An alcoholic extract of the term human placenta, when resolved into a protein/peptide component was found to show pigment and growth-inducing activity on B16F10 mouse melanoma cell in vitro. The protein/peptide component was studied in the dose range 0.01-100 mu g/ml. At a concentration of 0.1 mu g/ml, the growth of mouse melanoma was maximum, while the melanin formation was optimum at 50 mu g/ml. Furthermore, the fraction markedly changes cellular morphology. Presence of ET-1 (endothelin-1) and ACTH (adrenocorticotropic hormone), two important melanocyte response modifiers was detected in the fraction. The activity of the protein/peptide fraction, in respect of morphology, pigmentation and growth, when compared with a mixture of pure ET-1 and ACTH reconstituted, at a level that is present in it, was found to be significantly higher. [References: 33]