Metabolic control analysis of gene-knockout Escherichia coli based on the inverse flux analysis with experimental verification

摘要

Inverse flux analysis (IFA) was utilized to investigate the effect of gene-knockout on the metabolic flux distribution of Escherichia coli.In particular,it was shown from IFA that if pyk was knocked-out,then the flux through ppc and TCA cycle pathway increased,while the acetate production flux reduced.To verify this IFA result,we cultivated pykF~ E.coli and compared the fermentation data of E.coli K12.The enzyme activities of G6PDH,6PGDH,Pgi,Pfk,Pyk,Mez,MDH,Ppc,and Pyk were also measured.Those data well coincided with the IFA result.The effects of other gene-knockout such as pgi,zwf,etc.,were also investigated.From the present research,it was shown that the theoretical approach using computer shows some powers which needs careful attention.