Ascorbic acid stabilised copper nanoclusters as fluorescent sensors for detection of quercetin

摘要

In this report, green-emitting fluorescence copper nanoclusters (Cu NCs) were synthesized using ascorbic acid as reducing agent and protecting agent. The ascorbic acid capped Cu NCs (AA-Cu NCs) were characterized using fluorescence spectroscopy, UV-vis absorption spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The analysis data demonstrated that the AA-Cu NCs were highly dispersed with an average diameter of 2 nm. The as-prepared Cu NCs possessed good water solubility, excellent photostability and displayed excitation-dependent fluorescence characteristics. More importantly, the fluorescence intensity of AA-Cu NCs was linearly quenched in the presence of quercetin from 0.7 to 50 mu M and the detection limit (LOD) was 0.19 mu M. Finally, the fluorescence sensor was successfully employed to detect quercetin in bovine serum samples.