Some conventional eletrochemical sensor for simultaneous determination AA, DA and UA often use no-ble metal material owing to its electrocatalytic activity in eletrochemistry,but the cost of synthesis is too high, which limits its practical prospects. A nanohybrid based on multi-walled carbon nanotubes (MWCNTs) immobilized Cu nanoparticles (CuNPs@MWCNTs) for simultaneous electrochemical determination of ascorbic acid (AA), dopamine (DA) and uric acid (UA) was reported in this work. The CuNPs@MWCNTs-modified electrode showed a high selec-tivity towards the oxidation of AA, DA, and UA, and resolve their overlapped oxidation peaks into three well-defined peaks. Several important parameters that control the performance of the electrochemical sensor were investigated and optimized. Under pH4.0 phosphate buffered saline (PBS) with differential pulse voltammetry (DPV) method, the linear response ranges for the determination of AA, DA, and UA are 10 μmol/L~1430 μmol/L, 0.3 μmol/L~48.0μmol/L, 0.1μmol/L~86.7μmol/L in the co-existence systems, respectively, with the corresponding detection limits 3.3μmol/L, 0.1μmol/L, 0.03μmol/L (S/N=3), respectively. In addition, the proposed sensors provide an efficient and promising for simultaneous determination of AA, DA and UA selectively and sensitively and it cost much lower than those using noble metal nanoparticles, which has a good application development prospects in the biological sciences, clinical medicine and other fields.%一些传统的生化小分子多组分同时检测电化学传感器多是利用贵金属纳米材料在电化学中的催化活性,但是往往都是合成制备成本较高限制了其实用前景。该工作用比较廉价的铜纳米颗粒(CuNPs)修饰酸化的多壁碳纳米管(MWCNTs)制备纳米复合物(CuNPs@MWCNTs),代替贵金属纳米颗粒修饰碳材料,用比较低廉的成本构建了抗坏血酸(AA)、多巴胺(DA)和尿酸(UA)多组分同时检测的电化学传感器。CuNPs@MWCNTs修饰的电极对AA, DA和UA的氧化有很好的识别区分性,达到而且有些性能超过贵金属材料制备的传感器。在pH4.0的条件下用差分脉冲伏安法(DPV)传感器在三组分共存体系中检测抗坏血酸、多巴胺和尿酸的线性范围分别为:10μmol/L~1430μmol/L,0.3μmol/L~48.0μmol/L,0.1μmol/L~86.7μmol/L,AA、DA、UA的检测限依次是3.3μmol/L,0.1μmol/L,0.03μmol/L。因此,CuNPs@MWCNTs构建的传感器提供了一个比贵金属纳米颗粒成本较为低廉的进行灵敏快速有选择性的抗坏血酸、多巴胺和尿酸多组分同时检测的装置,在生物科学、临床医学等领域有很好的应用发展前景。
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