Optimization of medium using response surface methodology for l-DOPA production by Pseudomonas sp. SSA

摘要

L-3,4-Dihydroxyphenylalanine (l-DOPA) is the most potent drug used for treatment of Parkinsonism. Statistical optimization of nutritional parameters for the production of l-DOPA by Pseudomonas sp. SSA has been carried out using response surface methodology (RSM). Four most significant medium constituents identifiedby initial screening method of Plackett-Burman(PB) were glucose, peptone, L-tyrosine and CuSO4. In order to investigate quantitative effects of the four variables selected from PB design on l-DOPA production, Box-Behnken design was subsequently employed for further optimization. The medium having glucose 1.69 g l~(-1), peptone 1.61 g 1~(-1), L-tyrosine 4.11 g 1~(-1) and CuS04 0.03 g 1~(-1) was found to be optimum for maximum l-DOPA production. The optimization strategies used lead to a 6.27-fold increase in l-DOPA yield (3.251 ±0.12gl~(-1)) compared to non-optimized medium (0.572±0.1 g l~(-1))- l-DOPA produced was further characterized by spectroscopic techniques, such as HPTLC, HPLC and GC-MS.