Quantification of Prochlorperazine and Paracetamol Using High Performance Liquid Chromatography: Application to Tablets and Stability Studies

摘要

Tnis study reports a new stability indicating HPLC method using Spursil C18 column as stationary phase, and mixture of 0.1 M Na2HP04 and methanol (50:50 v/v) as mobile phase for the chromatographic determination of paracetamol and prochlorperazine in tablets and in bulk form. The linearity range is 250-750 ug/mL for paracetamol and 2.5-7.5 ug/mL for prochlorperazine. The limit of detection values are 2.650 ug/mL for paracetamol and 0.175 ug/mL for prochlorperazine. The minor values of the relative standard deviation (< 2.0 %) as well as good percent assay values (nearer to 100 %) confirm the high precision and accuracy of the present method. From the degradation study chromatograms found that there was no interference from degradants when paracetamol and prochlorperazine are quantified in tablets through the proposed method. A good agreement between results obtained and labeled claim for the determination of paracetamol and prochlorperazine in tablet samples demonstrates that the proposed method is appropriate to quantify paracetamol and prochlorperazine in tablet formulations.