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Evaluation of Phenolic Compounds in Commercial Fruit Juices and Fruit Drinks

机译:商业果汁和果汁饮料中酚类化合物的评估

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The total phenolic content of 13 commercially available fruit juices and juice drinks, selected to represent the most popular juice flavors in the United Kingdom, were analyzed using the Folin-Ciocalteu assay. Individual phenolic compounds were identified and quantified using HPLC-PDA-MS2. The catechin content and degree of polymerization of proanthocyanidins were also analyzed. Purple grape juice contained the largest number of individual phenolic compounds and also the highest concentration of total phenolics. The main components were flavan-3-ols, anthocyanins, and hydroxycinnamates, which accounted for 93% of the total phenolic content. In contrast, white grape juice, which contained principally hydroxycinnamates, had the lowest total phenolic content. Antioxidant activity was measured using the ORAC and FRAP assays, and the data obtained were in broad agreement with total phenol content. In view of the recent findings of the Kame project indicating that long-term fruit juice consumption can provide protection against Alzheimer's disease (Dai et al. Am. J. Med. 2006, 379, 464-475), it is suggested that the protective effects may be enhanced by consumption of a combination of juices rich in phenolics and containing a diverse variety of individual phenolic compounds, namely, juices derived from purple grapes, grapefruit, cranberries, and apples.
机译:使用Folin-Ciocalteu分析法分析了13种市售果汁和果汁饮料的总酚含量,这些饮料和果汁饮料被选为英国最受欢迎的果汁口味。使用HPLC-PDA-MS2对单个酚类化合物进行鉴定和定量。还分析了原花青素的儿茶素含量和聚合度。紫葡萄汁中含有最多的单个酚类化合物,并且总酚类化合物的浓度最高。主要成分是黄烷-3-醇,花青素和羟基肉桂酸酯,占总酚含量的93%。相反,主要包含羟基肉桂酸酯的白葡萄汁的总酚含量最低。使用ORAC和FRAP测定法测量抗氧化活性,所得数据与总酚含量基本一致。鉴于Kame项目的最新发现表明长期饮用果汁可以提供针对阿尔茨海默氏病的保护(Dai等人,Am。J. Med。2006,379,464-475)。食用富含酚类且含有多种单独酚类化合物的汁液(即源自紫葡萄,葡萄柚,蔓越莓和苹果的汁液)可以增强效果。

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