Cryopreservation of Sour Cherry (Prunus cerasus L. ) Using a Dormant Vegetative BUD Method

摘要

Five sour cherry varieties were successfully cryopreserved using cold hardy, dormant vegetative buds. Viability was estimated by chip budding to rootstock. Twig sections dried to moisture contents of approximately 25 % (fresh weight basis) survived exposure to ca. -160 deg C more successfully than those with higher moisture contents. Levels of survival observed were sufficient to initiate practical application of cryopreservation in sour cherry and in a test of 19 lines, 13 had survival rates exceeding 40 % after liquid nitrogen exposure. Further studies are needed to reduce variability in survival obtained after low temperature exposure and to define other factors that are important in retrieving viable bud and cambial tissues. The dormant, vegetative bud method is a simple system to preserve sour cherry, and can be used to screen lines that will require either modified, or entirely different, cryopreservation strategies.