Transmission of citrus yellow mosaic virus and its detection in mealybugs (Planococcus citri) by dot-blot hybridization.

摘要

A mealybug, Planococcus citri was identified as vector involved in transmission of citrus yellow mosaic virus (genus badnavirus) from citrus to citrus under glasshouse conditions in India, The vector transmit virus with acquisition access period of 24 h followed by inoculation access period of 24 h. Typical mosaic symptoms were observed on mealybug inoculated C. decumana plants similar to field symptoms. The virus was also detected in mealybugs feed on virus infected citrus seedlings for 24 h by dot-blot hybridization using 2.0 Kb cloned viral genome as DNA probe.