Fast and Sensitive Detection of Viral Targetsin Quantitative RT-PCR Using the Universal One-Step_qRT-PCR Master (ROX)

摘要

For _qRT-PCR assays it is a common procedure to performtwo individual work steps. First, RNA is transcribed into_cDNA, and then the target to be quantified is amplifiedseparately. This two-step approach is believed to result inhighest sensitivity of target detection. However, the sen-sitivity of nucleic acid amplification assays is determinedby multiple factors and differs from target to target. Incontrast, a one-step protocol in _qRT-PCR benefits from