Microsatellite marker based linkage map construction and mapping of granule bound starch synthase (GBSS) in rice using recombinant inbred lines of the cross Basmati370 / ASD16

摘要

In the present investigation, a framework linkage map of Basmati370 / ASD16 recombinant inbred population (F_9) based on microsatellite markers and candidate genes was constructed. Parental polymorphism survey with 471 SSR primer pairs identified about 357 pairs (markers) to be polymorphic between the parental lines. A subset of polymorphic markers was surveyed across the 182 RILs of the mapping population and the marker segregation data was used for the construction of a framework linkage map with 101 loci. Mapping of the candidate genes involved in nitrogen metabolism as well as in grain quality parameters was also taken up. Parental polymorphism survey with 22 candidate gene based primer pairs resulted in successful amplification of only nine primer pairs of which six were polymorphic and 3 were monomorphic between the parents. Three polymorphic primer pairs, each representing the genes encoding glutamate synthase (GOP5), starch branching enzyme (SBE12) and granule bound starch synthase (GBSSI)were surveyed across the RILs. Analysis of the marker segregation data from candidate gene primers resulted in successful mapping of only the gene GBSS (Wx) on the linkage group 6. The GBSS1 marker was positioned between the microsatellite markers RM4923and RM253 in the linkage group 6 of the framework genetic map.