We present a miniature microelectromechanical systems-based dual-axis confocal microscope capable of spatially coregistered fluorescence and reflectance imaging at multiple wavelengths. This device has a 10 mm diameter scan head with a 2 mm diameter tip for convenient use during surgery to guide tumor resection. The microscope has an adjustable focal depth of 20-200 micrometers and is capable of imaging with an axial resolution of 9 micrometers and in-plane resolution of 4 micrometers over a field of view of 450 X 450 micrometers. Simultaneous two-color imaging of individual optical sections is achieved by using a pair of grating-prism assemblies to compensate for chromatic dispersion in the 2 mm diameter gradient index relay lens at the distal tip of the device. Experimental measurements of the axial response of the microscope, as well as two-color images of a reflective bar target and fresh mouse brain tissues, demonstrate the performance of our device and its potential for multicolor in vivo optical sectioning microscopy.
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机译:我们提出了一种基于微型微机电系统的双轴共焦显微镜,能够在多个波长上进行空间共配的荧光和反射成像。该设备具有直径为10毫米的扫描头,尖端为2毫米,可在手术过程中方便地用于引导肿瘤切除。该显微镜具有20-200微米的可调焦深,并且能够在450 X 450微米的视场上以9微米的轴向分辨率和4微米的面内分辨率成像。通过使用一对光栅棱镜组件来补偿设备末端的直径为2 mm的梯度折射率中继透镜中的色散,可以实现单个光学部分的同时双色成像。显微镜的轴向响应的实验测量,以及反射条靶和新鲜小鼠脑组织的两色图像,证明了我们装置的性能及其在多色体内光学切片显微镜中的潜力。
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