We present a miniature micro-electro-mechanical systems (MEMS)-based dual-axis confocal microscope capable of spatially co-registered fluorescence imaging at multiple wavelengths. This device has a 10-mm diameter scan head with a 2-mm diameter tip for convenient use during surgery to guide tumor resection. The microscope has an adjustable focal depth from 20 – 200 microns and is capable of imaging with an axial resolution of 9 microns and in-plane resolution of 4 microns over a field of view of 450 × 450 microns. Simultaneous two-color imaging of individual optical sections is achieved by using a pair of grating-prism assemblies (GRISMs) to compensate for chromatic dispersion in the 2-mm diameter gradient-index (GRIN) relay lens at the distal tip of the device. Experimental measurements of the axial response of the microscope as well as two-color images of a reflective bar target and fresh mouse brain tissues demonstrate the performance of our device and its potential for multi-color in vivo optical-sectioning microscopy.
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